Fig. 6: TRIB3 abrogates the AKT-FOXO1 interaction.

a Supervised clustering of MCF7 cells transfected with CTRL-shRNA or TRIB3-shRNA using the log2-transformed values for genes in the FOXO1 upstream pathway, including AMPK, ERK, PI3K, p38, and JNK clustered genes expressed in all samples. Each column represents a separate case. Z-scores from IPA depicting the subnetwork gene expression differences between CTRL-shRNA and TRIB3-shRNA cases. The Z-score heat map depicts the five FOXO1 upstream subnetworks. Each row represents the data for the subnetwork indicated in the right margin. b, c TRIB3 inhibited the activity of AKT1 in BCCs. The indicated stable expression cell extracts were prepared and the levels of the indicated proteins were detected by immunoblotting. d, e The effects of the AKT1 agonist or antagonist for TRIB3-FOXO1-SOX2 axis in BCCs. The indicated cells were treated with the AKT1 agonist SC79 or the antagonist MK2206 for 24 h. Cell extracts were prepared and the levels of the indicated proteins were detected by immunoblotting. f TRIB3 inhibited the AKT1-FOXO1 interaction by binding to AKT1. HMLE cell extracts were IP with an anti-AKT1 Ab and blotted with an anti-TRIB3 or anti-FOXO1 Ab in the presence of the indicated concentration of the TRIB3-GST protein. Data are shown as the mean ± SEM; P > 0.05 was considered not significant (NS); *P < 0.05, **P < 0.01, and ***P < 0.001 compared with the vector or CTRL-shRNA group. Source data are provided as a Source Data file.