Fig. 5: Molecular events of root system establishment in white lupin.
From: High-quality genome sequence of white lupin provides insight into soil exploration and seed quality
a 8 developmental stages of cluster root development used for transcriptomic studies, showing the formation of numerous rootlets. b Comparative matrix layout of up-regulated genes in the 8 CR segments. Black dots indicate which sample parts (S0 to S7) are grouped and the number of up-regulated genes in the group is indicated on top of each bar. Set size indicates the total number of differentially expressed genes for each sampled fragment. Inserts: heatmap of the 42 genes over expressed in all CR regions (left) and miRNA expressed in the entire CR. c Partial phylogenetic tree of AP2/EREBP subfamily B-1 of Arabidopsis and white lupin orthologs, highlighting the 4 PUCHI orthologs in white lupin. d Left. Visualization of white lupin root system in 2D. Right. Number of lateral roots and cluster roots in GRAECUS (wild) vs. AMIGA (modern) accessions in the first 10 cm of the root system (n = 10). Bar is 5 cm. e Left. Expression pattern of 63 genes that are overexpressed in S1 region, which include LaPUCHI-1. Right. Relative expression of LaPUCHI-1 in AMIGA and GRAECUS in top lateral roots of 11-day-old plants. Box edges represent the 0.25 quantile and 0.75 quantile with the median values shown by bold lines. Whiskers extend to data no more than 1.5 times the interquartile range, and remaining data are indicated by dots. Source data underlying Fig. 5d, e are provided as a Source Data file.
