Fig. 6: White lupin seed protein and leaf alkaloid content.
From: High-quality genome sequence of white lupin provides insight into soil exploration and seed quality
a Grain appearance in GRAECUS (wild-type), Ethiopian landrace P27174 and cv. AMIGA. Bar is 0.5 cm. b Seed protein composition of AMIGA (DB, dark brown seeds; LB, light brown seeds), P27174 and GRAECUS (A and B: two independent extractions of proteins using Tris-SDS, separation in 12% SDS-PAGE). The bands extracted for MS/MS analysis are highlighted with red arrows. c Protein content quantification of each band extracted for each accession expressed in normalized gel volume. Data are presented as means ± SD, n = 2. d Abundance of the three major alkaloids in young leaf (3- and 5-leaf growth stage), stem and root tissues of the three accessions of L. albus as measured by LC-MS, and expression of the known alkaloid biosynthesis genes in young leaves as measured by qRT-PCR. n = 5 for AMIGA and n = 4 for P27174 and GRAECUS. e Putative biosynthetic pathway of tetracyclic quinolizidine alkaloids in lupins. Characterized steps and respective enzyme names are marked in red. More information about these analyses is described in Supplementary Note 9. LDC: lysine decarboxylase; CAO: copper amine oxidase; HLT: 13-hydroxylupanine O-tigloyltransferase. Data are presented as means ± SD, n = 4. Source data underlying Figs. 6b, d are provided as a Source Data file.
