Fig. 2: The function of T101 phosphorylation on AtTAA1 homologs and in TAA1 orthologs during evolution.
From: A phosphorylation-based switch controls TAA1-mediated auxin biosynthesis in plants
a–h Representative images of 7-day-old seedling in Col-0 a, wei8-3;tar2-1 b, wei8-3;tar2-2;tar1-1 segregated from wei8-3;tar2-2 crossed with wei8-3;tar1-1 F2 generation c, and 35S-TAA1T101D;wei8-3;tar2-1 d, e. Magnified picture of wei8-3;tar2-2;tar1-1 f and 35S-TAA1T101D;wei8-3;tar2-1 g, h. 54 out of 342 35S-TAA1T101D;wei8-3;tar2-1 seedlings (15.79%) from three independent lines (T2 generation) showed strong phenotype. Source data were provided in the source file. The result represents one of two replicates which showed a similar phenomenon. Arrows show asymmetric cotyledons and severe root defects. Scale bar, 0.25 cm a–e; 40 mm f–h. i Sequence alignment of TAA orthologs among Arabidopsis (At), Physcomitrella Patterns (Ppa), and Marchantia Polymorpha (Mp). Green colour column indicates the conserved residues as the AtTAA1 T101 site. j Phenotype of overexpressing MpTAAWT and MpTAAT101D in wild-type Marchantia (Tak-1) treated with 500 μM L-Kyn. Images denote representative thalli. Scale bar, 0.2 cm. k Quantification of the thallus size in j. Gammae from three independent lines of 35-MpTAAWT and two independent lines of 35-MpTAAT224D at 16 day old were quantified by thalli area. n denotes the number of independent thallus; two-sided t-test. P values were shown as indicated.
