Fig. 6: mTORC2 drives lipogenic and Glut4 gene expression through ACLY in mature brown adipocytes.
From: mTORC2-AKT signaling to ATP-citrate lyase drives brown adipogenesis and de novo lipogenesis
a Time-course western blots showing the indicated phospho-specific and total proteins in Rictor-iKOMBA or Acly-iKOMBA cells following acute and prolonged protein loss. b Direct acetyl-CoA measurements in Rictor-iKOMBA cells following acute Rictor loss (day 12) (n = 8 per group). c qRT-PCR analysis of the indicated genes following acute (day 12) and prolonged (day 14) Rictor loss (n = 6 per group). Rictor deletion was induced at day 8 during differentiation. d qRT-PCR analysis of the indicated genes Rictor-iKOMBA (n = 6 per group) stably expressing empty (EV), Myc-ACLY-WT, Myc-ACLY-S455A, or Myc-ACLY-455D constructs. Bar graphs represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, a represents ***p < 0.001 vs control (EV), and b represents ***p < 0.001 vs Rictor-iKOMBA (EV) cells. Statistical significance was calculated by using two-tailed unpaired Student’s t test (b) or two-way ANOVA with Sidak’s test multiple comparisons (c, d).
