Fig. 4: Nitrospinae and AOA growth rates calculated from 13C-bicarbonate and 15N-ammonium assimilation measured by nanoSIMS.
From: Single cell analyses reveal contrasting life strategies of the two main nitrifiers in the ocean
Nitrospinae 13C-bicarbonate assimilation rates were determined from water samples after the addition of 15N-ammonium and 13C-bicarbonate, and 15N-nitrite and 13C-bicarbonate. AOA data were acquired from the incubation with added 15N-ammonium and 13C-bicarbonate only and were taken from Kitzinger et al.31. Number of cells analyzed per population is indicated above each boxplot. Boxplots depict the 25–75% quantile range, with the center line depicting the median (50% quantile); whiskers encompass data points within 1.5x the interquartile range. Data of each measured cell are shown as points; horizontal position was randomized for better visibility of individual data points. Nitrospinae had significantly higher growth rates than AOA, as indicated by stars (one-sided, two-sample Wilcoxon test, W = 1984, p = 4.04 × 10−16 for growth based on 13C-bicarbonate assimilation and W = 1464, p = 3.32 × 10−12 for growth based on 15N-ammonium assimilation).
