Fig. 3: Cryo-electron tomogram of the tripartite droplets.
From: Tripartite phase separation of two signal effectors with vesicles priming B cell responsiveness
a A mixture of 1 μM of SLP651-330 and 1 μM of CIN85Δ57 together with 1 mM of SUVs was placed on a carbon grid and plunged frozen after incubation. The droplet shows a clear increase in concentration of vesicles toward its center with a steep fall-off at the edges. Golden spheres represent vesicles that were manually segmented for 3D-visualization in AMIRA 6.2 and processed using an in-house python script and Matlab. b Histogram of distances between vesicles with an average vesicle to vesicle distance of 4.92 ± 0.1 nm (mean ± SEM; n = 2349). c Histogram of the vesicle’s radii with a mean radius of 9.10 ± 0.03 nm (mean ± SEM; n = 1718). Source data are provided as a Source Data file.
