Fig. 6: Pyridoxine reduces dopaminergic neuron loss in the PD mouse model.

a–d Drd2^flox/flox and Drd2^hGFAPcKO mice were sacrificed after continuous infusion of 5 mg kg⁻¹ quinpirole or 5 mg kg⁻¹ pyridoxine for 7 consecutive days, and the striatum was dissected for further analysis. PKM2 dimer and tetramer formation analyzed by BN-PAGE and total PKM2 detected by immunoblotting with actin as a loading control (a). Gclc and Gclm mRNA detected by qRT-PCR (b). Immunoblotting with actin as a loading control (c) and GSH levels (d). Immunoblotting and qRT-PCR: three independent experiments; GSH assay: six independent experiments. e–h Drd2^flox/flox and Drd2^hGFAPcKO mice were used to generate an MPTP-induced PD mouse model (20 mg kg⁻¹ i.h., 5 d), which was treated by continuous infusion of quinpirole (5 mg kg⁻¹ i.p.) or pyridoxine (5 mg kg⁻¹ i.p.). Quantification of TH⁺ neurons (e). Striatal optical density (O.D.) measurements of TH-stained slices (f). Immunohistochemical staining of TH⁺ neurons (g). Immunohistochemical staining of TH in the nigrostriatal system (h). n = 6 mice per group. Scale bar, 200 µm. Data are presented as the mean ± s.e.m. **P < 0.01, ***P < 0.001, NS, not significant. Two-way ANOVA with Dunnett’s multiple comparisons test (b, d) or with Tukey’s multiple comparisons test (e, f). Source data are provided as a Source Data file.