Fig. 5: Effect of Fam13a on adipocyte differentiation of mouse SVFs isolated from SAT.
From: FAM13A affects body fat distribution and adipocyte function
a, b Fam13a (a) and Adipoq (b) mRNA expression, quantified by qRT-PCR, in freshly isolated SVF, primary mature adipocytes (floater), or during in vitro adipogenesis of cultured SVFs (D0, D4, and D10). (8-week-old male WT mice, n = 3 per group, n = 3 culture wells per animal) c mRNA expression of adipogenic markers (Pparg, Cebpa, Fabp4), measured by qRT-PCR during in vitro adipogenesis of cultured SVFs (D0, D4, D10). (8-week-old male WT or Fam13a KO mice when isolating SVFs, n = 6 per group, n = 3 culture wells per animal). d, e Oil-Red O staining of lipid droplets (d) and semi-quantification (e) in D10 differentiated adipocytes from SVFs. (n = 6 per group, pictures represent n = 6 independent experiments, n = 3 cultures/animal, ×10 magnification, scale bar=100 μm). f Intracellular triglyceride content in D10 differentiated adipocytes from SVFs. (n = 6 per group, n = 3 culture wells per animal). g Basal and insulin-stimulated [3H] glucose uptake in D10 differentiated adipocytes from SVFs. (n = 3 per group, n = 3 culture wells per animal). h Basal and insulin-stimulated [3H] glucose uptake in SAT explants. (8-week-old male mice, n = 3 per group, n = 3 ex vivo cultures per animal). Data are presented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA followed by Turkey’s multiple comparison test. Source Data are provided as a Source Data file.
