Fig. 4: Combined targeted therapy and immunotherapy for spontaneous PSCC in mice.
From: Effective combinatorial immunotherapy for penile squamous cell carcinoma
a Weight of resected penis from SA mice at the endpoint of the treatment course with single or combination therapies (n = 6, 3, 5, 3, 3, and 4, respectively). b Representative images of penises for the SA mice before and after the 1-month treatment course. c, d Quantification of IHC staining for CD11b and Ly6G, markers of tumor infiltrating granulocytic MDSCs, using SA tumor samples treated differently (independent IHC images for CD11b: n = 4, 5, 4, 9, 4, and 4, respectively; independent IHC images for Ly6G: n = 24, 10, 12, 12, 13, and 9, respectively). e Representative IHC images of Ly6G from the experiment in d. Scale bar 50 μm. f Quantification of IHC staining for Foxp3, marker of Tregs, using SA tumor samples treated differently (independent IHC images: n = 11, 6, 4, 3, 8, and 6, respectively). In a, box plots visualize the five-number summary of a data set (minimum, lower quartile, median, upper quartile and maximum). In c, d, and f, all data are shown with the line representing the median. #P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two-sided Student’s t test.
