Fig. 1: Overview of the applied Omics approaches.

Viral gene expression was analyzed in primary human fibroblasts (HFF). The total RNA-seq, 4sU-seq and ribosome profiling data were recently published¹¹. To comprehensively identify transcription start site (TiSS), we performed cRNA-seq² and dRNA-seq¹² as well as RNA-seq on subcellular RNA fractions from mock, wild-type and ΔICP27-infected cells. For all of these, two biological replicates were performed. Furthermore, we incorporated the recently published transcripts originating from PacBio¹³ and MinION¹⁴ sequencing data. Translation start site (TaSS) profiling was performed by ribosome profiling following treatment of cells for 30 min with either Harringtonine or Lactimidomycin². Proteome analysis included two whole-proteome datasets using SILAC and label-free mass spectrometry. The available timepoints and conditions are indicated by stars.