Fig. 2: vHMM-HA has superior cytoprotective properties.
From: Naked mole-rat very-high-molecular-mass hyaluronan exhibits superior cytoprotective properties
a Pulse-field gel electrophoresis image of NSF-HA and MSF-HA. The experiment was repeated once with similar result. b NSF-HA, but not MSF-HA, suppresses oxidative stress-induced cell death in IMR90 cells. The percentages of live cells were measured by Annexin-V/PI staining 1 day after 1 h of 3 mM tBHP-treatment (n = 3). Cells were pre-incubated for 6 h with 20 μg/ml NSF-HA, MSF-HA, or PBS before tBHP-treatment. c NSF-HA, but not MSF-HA, suppresses oxidative stress-induced growth arrest in IMR90 cells (n = 4). Cells were pre-incubated for 6 h with 20 μg/ml of NSF-HA, MSF-HA, or PBS and then HA was removed and cells were exposed to 200 μM tBHP for 1 h on day 0 and day 1. d Pulse-field gel electrophoresis image of cNSF-HA and fNSF-HA. The experiment was repeated once with similar result. e cNSF-HA, but not fNSF-HA, suppresses oxidative stress-induced cell death in IMR90 cells. The percentages of live cells were measured by Annexin-V/PI staining 1 day after 1 h of 3 mM tBHP-treatment (n = 3). Cells were pre-incubated for 6 h with 20 μg/ml cNSF-HA, fNSF-HA, or PBS before tBHP-treatment. f cNSF-HA, but not fNSF-HA, suppresses oxidative stress-induced growth arrest in IMR90 cells (n = 4). Cells were pre-incubated for 6 h with 20 μg/ml cNSF-HA, fNSF-HA, or PBS and then HA was removed and cells were exposed to 200 μM tBHP for 1 h on day 0 and day 1. g The heatmap shows the CD44-normalized abundances of 99 CD44 co-immunoprecipitated proteins that are differentially associating with CD44 in CD44-overexpressing IMR90 cells. Cells were incubated for 6 h with 20 μg/ml cNSF-HA, fNSF-HA, or PBS. The experiment was done in triplicates. (h) Western blots showing the abundances of CD44-FLAG co-immunoprecipitated CD44-YFP in IMR90 cells overexpressing CD44-FLAG and CD44-YFP (n = 9). Cells were incubated for 6 h with 20 μg/ml cNSF-HA, fNSF-HA, or PBS. i Quantification of Western blots shown in (h) by Image J. Error bars are presented as mean ± SD values. *p < 0.05 (one-way ANOVA with post-hoc Dunnett’s two-tailed test).
