Fig. 5: MAGL modulates GSC self-renewal by regulating PGE2. | Nature Communications

Fig. 5: MAGL modulates GSC self-renewal by regulating PGE2.

From: ARS2/MAGL signaling in glioblastoma stem cells promotes self-renewal and M2-like polarization of tumor-associated macrophages

Fig. 5

a, b Representative ICC images of PGE2 and β-catenin in GSCs (528 and X01 cells) infected with a shMAGL-expressing lentiviral or shCtrl construct. Scale bar, 50 µm. c, d Immunoblot (IB) analysis of MAGL and β-catenin in fractionated nuclear or cytosolic lysates from 528 cells and X01 cells infected with a shMAGL-expressing lentiviral or shCtrl construct. Lamin B and β-actin were used as markers for nucleus and cytoplasm, respectively. e IB analysis of β-catenin in 578 cells treated with different concentrations of PGE2 (0.1, 1, and 10 μM). Vinculin was used as a loading control. f, g Sphere-formation assays performed using GSCs (578 cells) treated with PGE2 alone, PGE2 and ICG-001 (10 μM), or vehicle. Images are representative of three independent experiments. Scale bar, 50 µm. The graph shows the average number of spheres >20 μm in diameter. All error bars represent mean ± SEM (n = 3). **P < 0.01, t-test. h, i Limiting dilution assays (LDAs) performed using GSCs (528 and X01 cells) treated with ICG-001 (10 μM) or vehicle. (n = 24, **P < 0.01, t-test).

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