Fig. 1: EEF1A1 is deacetylated by HDAC1/2 in SCs.

a Sox10 Western blot and quantification normalized to GAPDH showing low Sox10 levels at 1dpl in crushed as compared to contralateral sciatic nerves of adult mice. Paired two-tailed Student’s t-tests, p value = 0.008938, n = 8 animals per group. b, c Co-immunofluorescence of Ac-eEF1A and S100 (SC marker) and DAPI (nuclei) labeling (b) and immunoprecipitation (IP) of eEF1A1 and Western blot of Ac-eEF1A (c) in sciatic nerves of P4 control (Ctrl) and DhhCre;Hdac1;Hdac2 knockout (dKO) mice showing increased levels of Ac-eEF1A in the absence of HDAC1/2 in SCs. n.s. = non-specific. d Western blot of total eEF1A1 and GAPDH (loading control) in sciatic nerves of P4 Ctrl and dKO mice and quantification of eEF1A1 levels normalized to GAPDH levels showing no significant difference between the two groups. Paired one-tailed Student’s t-tests, p value = 0.297774, n = 4 animals per group. e IP eEF1A1 followed by Western blot of Ac-eEF1A in nuclear and cytoplasmic fractions of primary SCs cultured under de-differentiating conditions and treated with the HDAC1/2 inhibitor mocetinostat (Mocet.) or its vehicle (Veh.) for 3 days. Lamin A/C (nuclear marker), GAPDH (cytoplasmic marker) and eEF1A1 Western blots on lysates used for IP show the inputs. f Immunofluorescence of Ac-eEF1A and DAPI labeling in primary SCs cultured under de-differentiation conditions and treated with mocetinostat or its vehicle for 3 days. Arrowheads (b) and arrows (f) show SC nuclei. Z-series projections (b) or single optical sections (f) are shown. b, f Representative images of 3 different animals per group or of 3 independent experiments are shown. b, d Data are presented as mean values ± SEM. Scale bars: 10 µm. Source data are provided as a Source Data file.