Fig. 2: Sox10 re-localizes to the cytoplasm with Ac-eEF1A1 in de-differentiated SCs.

Immunofluorescence of Ac-eEF1A (a) and Sox10 (c), and DAPI (nuclei) labeling in differentiated and de-differentiated SCs. b Immunoprecipitation (IP) of eEF1A1 or GFP (control) carried out on the same pool of two adult mouse crushed or unlesioned contralateral sciatic nerves at 3dpl and Western blot of Ac-eEF1A followed by HDAC2. EEF1A1 and GAPDH Western blots on lysates show the input, n = 3 (6 animals). The graph shows the quantification of Ac-eEF1A levels (measured on a longer exposure to obtain a value for the contra IP eEF1A1) normalized to eEF1A1 input. Data are presented as mean values ± SEM. Paired two-tailed Student’s t-tests, p value = 0.03376. IP Sox10 or GFP (d) or IP eEF1A1 (e) followed by Ac-eEF1A (d) or HDAC2 (e) Western blot in nuclear and cytoplasmic fractions of primary SCs cultured under de-differentiating conditions and treated with the HDAC1/2 inhibitor mocetinostat or its vehicle for 3 days. Lamin A/C (nuclear marker), GAPDH (cytoplasmic marker), Sox10 and eEF1A1 Western blots on lysates used for IP show the inputs. Dashed lines indicate that samples were run on the same gel but not on consecutive lanes. Arrows point to SC nuclei (a, c) and arrowheads (c) to SC cytoplasm. a–e Representative images of 3 independent experiments are shown. Scale bars: 10 µm. Source data are provided as a Source Data file.