Fig. 6: PDZD8-Rab7 mediated ER-LE contact site can form during endosomal maturation. | Nature Communications

Fig. 6: PDZD8-Rab7 mediated ER-LE contact site can form during endosomal maturation.

From: PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria

Fig. 6

a The lysosomal marker LAMP1 is not detected in PDZD8-associated Rab7-lableled endosomes in cells Live imaging of U2OS cells co-expressing PDZD8-GFP, BFP-Rab7, and LAMP1-mCherry. Mitochondria are labeled with MitoTracker DeepRed. Inset corresponds to magnified area. Scale bar: 10 μm. b The early endosomal marker, Rab5, is not detected in PDZD8-associated Rab7-labeled endosomes in cells. U2OS cells expressing PDZD8-GFP (in green), BFP-Rab7 (in blue), and mCherry-Rab5 (in red). Mitochondria are labeled with MitoTracker DeepRed (in white). Scale bar: 10 μm. c The Rab7 effector, WDR91 localizes to PDZD8-labeled late endosomes. U2OS cells co-expressing PDZD8-GFP (in green), BFP-Rab7 (in blue), and mCherry-WDR91 (in red). Mitochondria are labeled with MitoTracker DeepRed (in white). Inset corresponds to magnified area. Scale bar: 10 μm. d Schematic representation of the PDZD8/Protrudin-Rab7 ER-endosome membrane contact site.

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