Fig. 4: Hth regulates the expression of Dscam1.

Lateral views of L3 larval brains showing the NB (a, b, d) and neuron layers (c, e; see Fig. 1b). Dscam1 (white in a–c, magenta in d, e). a Dscam1 expression overlaps with Hth (green) in NEs, and with Hth and Dpn (magenta) in NBs. b, c Background level of Dscam1 signal in hth mutant clones visualized by the absence of GFP (green; arrows) compared with the control cells (arrowheads). Pixel intensity of Dscam1 signal was uniformly enhanced throughout the image (b). d, e Ectopic Dscam1 upregulation in clones expressing hth visualized by GFP (green; arrows) under the control of Ay-Gal4. Ncad (blue). The areas indicated by the dotted boxes are enlarged in the right panels. Scale bars indicate 20 μm. f–i Quantification of signal intensity in the boxes in (b–e). Intensities in the dotted boxes are plotted with dotted lines. f, g Dscam1 signal is reduced in GFP-negative hth mutant clones (b, c). h, i Dscam1 signal is enhanced in GFP-positive hth expressing clones (d, e). Background signal was subtracted for Dscam1 (f, h). Experiment was independently repeated at least three times with similar results (a–e). Source data are provided as a Source Data file (e, g, h, i).