Fig. 5: Loss of Dscam1 impairs lineage-dependent repulsion. | Nature Communications

Fig. 5: Loss of Dscam1 impairs lineage-dependent repulsion.

From: Dscam1 establishes the columnar units through lineage-dependent repulsion between sister neurons in the fly brain

Fig. 5

Neurons of the same lineage are visualized by elav-Gal4 MARCM clones (GFP in white). Ncad (blue) visualizes the neuropil structure and columns. Projection patterns of neurons of the same lineage in control (a) and Dscam1 mutant clones (b). Lateral views of L3 larval brains showing the neuron layer (see Fig. 1b). Wide spread tangential projections found in M0 layer in control clones (a) are suppressed in Dscam1 clones (b; arrowheads). The neurons innervate the medulla layer following M0 layer. c Quantification of the distance between neurites of the same lineage. Control: n = 8. Dscam1 mutant: n = 10, Center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range. Average projection distances are 49.2 and 17.45 μm, respectively. SD are 14.8 and 8.09, respectively (two-sided t test, P = 0.00043). d, e Column morphology of 48 h APF pupal medulla showing the M1–2 layers. Control (d) and Dscam1 mutant clones (e). Regular column morphology is disrupted in and around Dscam1 mutant clones (arrows). f Classification of column morphology. In contrast to normal columns, abnormal columns are classified into irregular, fused and unclear columns. g Quantification of column morphology. Control: 87 columns from 3 brains. Dscam1 mutant clones: 136 columns from 3 brains (Fisher exact test, normal P = 8.4 × 10−47, unclear P = 7.23 × 10−19, irregular P = 7.18 × 10−12, fused P = 0.00046). h, j Tangential migration of neuronal cell bodies of the same lineage at 24 h APF. Dorsal views showing control (h) and Dscam1 mutant clones (j). i, k Spatial distribution of the cell bodies in the medulla cortex in (h, j) are quantified. The wide spread tangential distribution in control (h, i; SD = 42 μm, n = 3) is suppressed in Dscam1 mutant clones (j, k; SD = 24 μm, n = 3, two-sided t test, P = 0.0022). l Schematic representation of the optic lobe at 24 h APF. Scale bars indicate 20 μm in (a, b, d, e, h, j) and 5 μm in (f). Experiment was independently repeated at least three times with similar results (a, b, d, e). Source data are provided as a Source Data file (c, g, i, k).

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