Fig. 6: Loss of Dscam1 isoform diversity leads to defects in column formation. | Nature Communications

Fig. 6: Loss of Dscam1 isoform diversity leads to defects in column formation.

From: Dscam1 establishes the columnar units through lineage-dependent repulsion between sister neurons in the fly brain

Fig. 6

a Schematic representation of Dscam1 gene in wild type and Dscam1 single-isoform mutant backgrounds. Column morphology of 48 h APF pupal medulla visualized by Ncad (blue) in the M1–2 (top) and M9–10 (bottom) layers in control (b) and Dscam1 single mutant backgrounds (c; Dscam123/Dscam13.31.8). Scale bars indicate 20 μm. Experiment was independently repeated at least three times with similar results (b, c). d Schematic representation of the optic lobe at 48 h APF. (e) Quantification of column morphology in the top layer. Control: 74 columns from 5 brains. Single-isoform mutant: 143 columns from 5 brains (Fisher exact text, normal P = 2.11 × 10−40, fused P = 5.4 × 10−17, irregular P = 0.33, unclear P = 0.21). Source data are provided as a Source Data file.

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