Fig. 4: The absence of RIPK3 in macrophages prevents AAA development induced by ALI. | Nature Communications

Fig. 4: The absence of RIPK3 in macrophages prevents AAA development induced by ALI.

From: Lung-derived HMGB1 is detrimental for vascular remodeling of metabolically imbalanced arterial macrophages

Fig. 4

Aneurysm incidence (a), representative photomicrographs of aorta (b), and aneurysm severity (c) in Ripk3+/+→WT and Ripk3−/−→WT bone marrow transplant chimeras treated with Ang II in the presence of ALI or not. n = 4 (Ripk3+/+→WT, Ripk3−/−→WT, and Ripk3−/−→WT + ALI) and 5 (Ripk3+/+→WT + ALI). Representative color Doppler ultrasound images of abdominal aorta and aortic diameter measurement (arrows) on M-mode screenshots (d), time course of aortic diameter (e), and maximum aortic diameter (f) of mice as indicated. n = 4 (Ripk3+/+→WT, Ripk3−/−→WT, and Ripk3−/−→WT + ALI) and 5 (Ripk3+/+→WT + ALI). *P = 0.0453, **P = 0.002. Verhoeff-Van Gieson staining and elastin degradation score (g). Arrows point to elastin breaks. n = 4 per group. *P < 0.05. (h) Representative microscopy images of CD68 and quantification of aortic sections of mice as indicated (scale bar = 20 µm). n = 3 (Ripk3+/+→WT) and 4 (Ripk3+/+→WT + ALI, Ripk3−/−→WT, and Ripk3−/−→WT + ALI). *P < 0.05, **P < 0.01, ***P < 0.001. Data is presented as mean, error bars represent s.e.m. P values were calculated using two-tailed unpaired t-tests (e) or one-way ANOVA (f–h).

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