Fig. 4: Inhibition of SARS-CoV-2 entry by Fc-fused sdAbs. | Nature Communications

Fig. 4: Inhibition of SARS-CoV-2 entry by Fc-fused sdAbs.

From: Humanized single ___domain antibodies neutralize SARS-CoV-2 by targeting the spike receptor binding ___domain

Fig. 4

a Representation of the human IgG1 Fc-fused sdAbs in this study. SdAb-Fc fusion construction generates a bivalent molecule with an approximate molecular weight of 80 kDa. b Homology modeling of the bivalent 5F8-Fc molecule with SWISS-MODEL server (https://swissmodel.expasy.org)23. The template structure for 5F8 modeling was based on a humanized camelid sdAb in the PDB database (3EAK). The structure is depicted as cartoons and colored with secondary structure. Three CDRs, hinge region and Fc were indicated. c Five Fc-fused sdAbs were analyzed by Western blot with gradient SDS-PAGE in reducing (with β-mercaptoethanol) or non-reducing (without β-mercaptoethanol) condition. d Summary of EC50 value of Fc-fused sdAbs neutralization against SARS-CoV-2pp. EC50 fold increases versus the corresponding monovalent sdAbs were calculated.

Back to article page