Fig. 2: Photoswitchable performance of AzTax. | Nature Communications

Fig. 2: Photoswitchable performance of AzTax.

From: Photoswitchable paclitaxel-based microtubule stabilisers allow optical control over the microtubule cytoskeleton

Fig. 2

a Photostationary state UV–Vis absorption spectra of AzTax3MP under a range of cell-compatible wavelengths similar to microscopy laser lines. b, c Resazurin antiproliferation assays of AzTax highlight their structure- and light-dependent cell cytotoxicity. HeLa cells, 40 h incubation in dark conditions (all-E) or under pulsed illuminations with low-power LEDs (75 ms per 15 s near-UV at < 1 mW cm-2; lit = ~80% Z) (dose-response curves are fitted to the means (shown with s.d.) of 3 independent biological experiments). d A cell-free assay for polymerisation of purified tubulin comparing the MT stabilisation activity of docetaxel, all-E- and 360 nm-lit-AzTax3MP (all 10 µM) shows light-specific promotion of polymerisation by Z-AzTax3MP, matching the trend observed in cellular assays.

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