Fig. 5: Increase of thermodynamic stability of BsCspB due to the presence of c = 120 g/L MC.
From: All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy
The increase in the transition midpoint, ΔCM, obtained for amide protons is mapped onto the three-dimensional structure of BsCspB (PDB ID: 1NMG) applying Dex20 (left) or PEG1 (right). The following color code has been used: 0.2 M ≤ ΔCM ≤ 0.3 M in light orange, 0.4 M ≤ ΔCM ≤ 0.6 M in orange and 0.7 M ≤ ΔCM ≤ 0.9 M in bright orange whereas residues colored in gray report on lack of experimental data. Numerical values for ΔCM obtained under these experimental conditions can be found in Fig. 2c, d and Supplementary Table 2.
