Fig. 4: Altered neurogenesis in the developing POMC neurons with ciliary dysgenesis.
a The numbers of tdTomato-labeled POMC neurons in the ARH of POMC-cre;;KIF3Af/f;;tdTomato mice compared with POMC-cre;;tdTomato littermates (n = 5 for POMC-cre;;IFT88f/f mice at both ages, n = 4 for IFT88f/f mice at 3 weeks, n = 3 for IFT88f/f mice at 8 weeks). Measurements were conducted at the indicated ages. *p = 0.0367. Scale bars: 100 μm. 3V third cerebroventricle. b The numbers of β-endorphin-expressing POMC neurons in the ARH of POMC-cre;;IFT88f/f mice and IFT88f/f littermates at the indicated ages (n = 5 for POMC-cre;;IFT88f/f mice and n = 6 for IFT88f/f mice). **p = 0.002. Scale bars: 50 μm. c 5-Bromouridine (BrdU) and POMC (β-endorphin) double staining showing the reduced POMC neurogenesis during E10.5–E12.5 in POMC-cre;;IFT88f/f mice (n = 4 for POMC-cre;;IFT88f/f mice, n = 3 for IFT88f/f mice). The experimental schedule was presented. Arrowheads indicate BrdU+ POMC neurons. *p = 0.023. Scale bars: 50 μm. d BrdU/β-endorphin double staining showing the increased neurogenesis of POMC neurons in POMC-cre;;IFT88f/f mice during the post-weaning period (3–6 weeks) (n = 5). Arrowheads indicate BrdU+ POMC neurons. *p = 0.027. Scale bars: 50 μm. Data are presented as the mean ± SEM values. Statistics were performed using two-sided Student’s t test (a–d). ns not significant.
