Fig. 2: miRNA sensing with high specificity for the whole region of miRNA.
From: Quantification of purified endogenous miRNAs with high sensitivity and specificity
a Sequences of let-7a and its single-nucleotide mutations (red) at seed, mid, and tail regions. b Representative fluorescence intensity time traces showing binding of let-7a probes to let-7a_m1 (top), let-7a_m2 (middle), and let-7a_m3 (bottom), respectively. c Duty cycles of let-7a probes on wild-type let-7a and its mutants. d The sequences of let-7 family miRNAs. e Positive rates (either true: diagonal, or false: off-diagonal) of let-7 family miRNAs measured using Ago-FISIH. f The sequences of let-7 miRNAs with varying U-tail length. g Positive rates (either true: diagonal, or false: off-diagonal) of let-7 miRNAs with varying U-tail length measured using Ago-FISH. To obtain each data point of c, e, and g, three or more independent experiments were conducted and at least 300 molecules in total were analyzed. The criteria of 5% duty cycle was used to make the tables. Data are mean ± SEM. For all statistical analyses of miRNA detection in this work, 20 min imaging time was used. Source data are available in the Source data file.
