Fig. 2: Cryo-EM structures of three different forms of the Tko RNAP.
From: Direct binding of TFEα opens DNA binding cleft of RNA polymerase
a Cryo-EM density maps of the apo-form (left), RNAP–TFEα binary complex (center), and RNAP–TFEα–DNA ternary complex (right). Domains of RNAP and DNA are indicated and variably colored. The DNA used for preparing the ternary complex is shown (right). DNA bases shown in gray are disordered in the structure. Note that the cryo-EM maps of RNAP–TFEα binary complex and RNAP–TFEα–DNA ternary complex are the composite maps produced from globally refined maps and focused refinements on the region containing TFEα, stalk and cleft domains. b Ribbon models of the apo-form (left), binary complex (center) and ternary complex (right) in the same color and orientation as in (a).
