Fig. 7: Bt1762-63 binding to medium-chain fructo-oligosaccharide (FOS).
From: Insights into SusCD-mediated glycan import by a prominent gut symbiont
a Native mass spectrum of Bt1762-63 bound to FOS T159 fraction (~DP9) revealed the presence of both bound and unbound SusCD monomers, but only FOS-bound SusC2D2 dimers. The inset shows the spectrum of the FOS T159 fraction. b Spectrum of Bt1762-63 incubated with different concentrations of FOS T159. Shown are individual charge states of the monomer (+23) and of the dimer (+32). Up to two and four FOS molecules (mainly 8–10 fructose units) were found to bind to SusCD and SusC2D2, respectively. Masses of all species observed are listed in Supplementary Table 6.
