Fig. 5: The SARS-COV-2 N protein forms highly dynamic condensates in cells whose liquidity are likely regulated by phosphorylation. | Nature Communications

Fig. 5: The SARS-COV-2 N protein forms highly dynamic condensates in cells whose liquidity are likely regulated by phosphorylation.

From: The SARS-CoV-2 nucleocapsid phosphoprotein forms mutually exclusive condensates with RNA and the membrane-associated M protein

Fig. 5

a Representative fluorescence images of Clover-tagged N, N11SD, N14SA, and N∆SR (∆176–206) in U2OS cells. b Fraction of cells showing N protein condensates when expressing Clover-tagged N, N11SD, N14SA, or N∆SR. See Supplementary Fig. 6 for analysis with SRPK and GSK3 kinase inhibitors. N represents number of cells counted in each experiment. c Representative example of FRAP analysis of Clover-tagged wild-type N protein. Enlarged pictures are fluorescence images of one condensate after partial photobleaching (1) and one after full photobleaching (2). Scale bar, 5 µm for original image and 1 µm for enlarged images. d Mean fluorescence intensity plot for FRAP analysis of Clover-labeled N (n = 7 droplets), N11SD (n = 9 droplets), N14SA (n = 13 droplets), and N∆SR (n = 10 droplets) in cells. See Supplementary Fig. 5 for full images of NWT, N11SD, N14SA, and N∆SR. Mean average data are normalized to the average intensity of a particle before photobleaching and are represented as mean ± standard deviation from the recovery curves.

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