Fig. 6: Human receptor mutations which disrupt the receptor–ligand interface.
From: Structures of mouse and human GITR–GITRL complexes reveal unique TNF superfamily interactions
a mIL-2 secretion upon hGITRL stimulation with hGITR cell lines expressing WT, F106S, or G109D variants (n = 3; independent experiments performed six times). b Confocal imaging of WT cells fixed before staining with fluorescently labeled hGITRL-sc trimers shows diffuse surface GITR. c WT and F106S mutant hGITR expressing cell lines fixed after staining with fluorescent hGITRL-sc trimers reveals discrete GITR puncta on WT cell surface, but no hGITRL-sc binding of F106S cells. This imaging experiment was performed once, cells plated in duplicate. Source data are provided as a Source Data file.
