Fig. 4: MINFLUX single-molecule tracking at ~100 µs temporal sampling. | Nature Communications

Fig. 4: MINFLUX single-molecule tracking at ~100 µs temporal sampling.

From: MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope

Fig. 4

a Fluorescence intensity over time for a ~3-s excerpt of tracking DPPE-ATTO 647N lipid diffusion (at room temperature) in a supported lipid bilayer. b Histogram of photons per localization for the data shown in (a). c Duration of individual localizations. d Histogram of the standard deviations in the lateral displacements Δx and Δy of consecutive localizations, providing a conservative measure on the precision of individual localizations. e xy trajectory color coded to indicate the passage of time. f Enhanced temporal acquisition, sampling at 8.577 kHz (mean of 117 µs per localization). g, h Data as in (c, d) for the faster single-lipid tracking experiment. Source data are provided as a Source Data file. Scale bars: 200 nm (e, f).

Back to article page