Fig. 5: 3D MINFLUX imaging. | Nature Communications

Fig. 5: 3D MINFLUX imaging.

From: MINFLUX nanometer-scale 3D imaging and microsecond-range tracking on a common fluorescence microscope

Fig. 5

a Individual TCPs for iterative 3D MINFLUX are constructed from an octahedron-shaped coordinate set. Coordinates that deviate from the nominal focal plane are addressed by defocusing via a deformable mirror. b Lateral and c axial precision of Nup96-SNAP localizations, inferred from single-molecule fluorescence event aggregates of ~1200 photons. d Histogram of the distance between individual localizations of a single fluorophore and its mean position estimate. e Example of raw data and f rendering of a pore compare Supplementary Movie S1 for a large field of view. Panels (b–d) display pooled data from these data sets. g Clathrin visualized by SNAP labeling of the clathrin light chain in HeLa cells. The region demarcated by the white lines has an extent of 2.2 × 1.1 µm2. All SNAP-tags were labeled with Alexa Fluor 647. Source data are provided as a Source Data file. Scale bars: 4 nm (d), 100 nm (e, f), 200 nm (g).

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