Fig. 1: SPA and its derivative LM-2I inhibit breast cancer proliferation and growth. | Nature Communications

Fig. 1: SPA and its derivative LM-2I inhibit breast cancer proliferation and growth.

From: Naturally-occurring spinosyn A and its derivatives function as argininosuccinate synthase activator and tumor inhibitor

Fig. 1

a Chemical structures of SPA (1). b Crystal violet assay for the effects of SPA on breast cancer cell proliferation. IC50 values were calculated with SPSS 18.0 (mean ± s.d., n = 3 biologically independent experiments). c Representative microscope images of colony formation for different breast cancer cell lines treated with SPA. Scale bars are 40 µM. d Chemical structures of LM-2I (2). e Crystal violet assay for the effects of LM-2I on breast cancer cell proliferation. IC50 values were calculated with SPSS 18.0 (mean ± s.d., n = 3 biologically independent experiments). f Representative microscope images of colony formation for different breast cancer cell lines treated with LM-2I. Scale bars are 40 µM. MDA-MB-231 cells were inoculated subcutaneously into the right flank of nu/nu female mice. When the average tumor size reached about 100 mm3, mice were treated with ether vehicle, SPA (10 mg/kg/day) or LM-2I (5 mg/kg/day). Tumor sizes (g) and mouse body weight (i) were measured every 2 days. 28 days later, mice were sacrificed and tumors were dissected and weighted (h). Data represent mean ± s.d. (n = 8 mice per group). *P < 0.05, ***P < 0.001, NS: not significant (P > 0.05) from two-tailed unpaired Student’s t tests.Source data are provided as a Source Data file.

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