Fig. 3: tmFRET between the N-terminal end of the S5 helix and A′ helix of the C-linker.
From: Electromechanical coupling mechanism for activation and inactivation of an HCN channel
a Cartoon showing tmFRET between C369Anap in the S5 helix and Cu2+-TETAC attached to Y477C in the A′ helix. b Cartoon showing tmFRET between W355Anap in the S4 helix and Cu2+-TETAC attached to Y477C in the A′ helix. c FRET efficiencies of spHCN-C369Anap, Y477C channels measured in four conditions: the presence and absence of cAMP, and at 0 and −100 mV; n = 7 independent patches for 0 mV conditions and n = 4 independent patches for −100 mV conditions. **p < 0.001; p = 0.1 between no cNMP, 0 mV and no cNMP, −100 mV conditions. d FRET efficiencies of spHCN-W355Anap, Y477C channels measured in four conditions: the presence and absence of cAMP, and at 0 and −100 mV; n = 3 independent patches for cAMP and n = 4 independent patches for no cNMP. **p < 0.001 and *p < 0.05. Data shown are mean ± s.e.m.; source data are provided in the Source Data file.
