Fig. 5: Comparison of the measured distances from tmFRET and the corresponding distances from Rosetta models. | Nature Communications

Fig. 5: Comparison of the measured distances from tmFRET and the corresponding distances from Rosetta models.

From: Electromechanical coupling mechanism for activation and inactivation of an HCN channel

Fig. 5

a–d Comparison of the distances of FRET pairs measured from tmFRET (circle, mean ± s.e.m, n = 3–7) using the FCG relation in Fig. 4b and mean Cβ–Cβ distances of these FRET pairs in the 17 top scoring Rosetta models (square) based on the experimentally-determined distance constraints in four states: resting-cAMP (a), activated (b), resting-apo (c), and inactivated (d). The standard deviation of the Cβ–Cβ distances from the top 17 scoring Rosetta models for each state was less than 0.3 Å.

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