Fig. 10: Characterization of KHDRBS1-LCK-driven PTCL in mice.

a Kaplan–Meier survival curve after transplantation with HSPC transduced with empty pMIG vector (n = 5) or pMIG-KHDRBS1-LCK (n = 5). Log-rank p value was obtained from a two-sided Chi-square test. b Representative H&E stains of lymph nodes from mice who were transplanted with HSPC transduced with empty vector (n = 5) or KHDRBS1-LCK (n = 1). Low magnification images are in the top row, scalebars represent 200 µm. High magnification images are in the bottom row, scalebars represent 50 µm. c Representative flow cytometry plots for cell suspensions from the lymph nodes of empty vector mice (representative for n = 5) and the KHDRBS1-LCK mouse (n = 1). d Gating strategy for CD4⁺CD8⁺ double positive cells and CD4⁺CD8⁻ single positive cells on thymic cell suspcensions from mice with empty-vector-transduced cells and GFP⁺ cells in KHDRBS1-LCK induced PTCL (left). Quantification of intracellular TdT with intracellular flow cytometry. e Western blot for phospho-LCK (Tyr394) and total LCK in spleen lysates from mice transduced with HSPC transduced with empty vector or KHDRBS1-LCK-induced PTCL.