Fig. 2: The uterine and vaginal microbiome of healthy women and women with chronic endometritis.

HV, DV, HU, and DU represent healthy vagina, endometritis vagina, healthy uterus, and endometritis uterus, respectively. a Microbial diversity of the uterine and vaginal microbiome in women with different health statuses. Box-plot elements are defined as: center line, median; box limits, upper and lower quartiles; whiskers, 1.5× interquartile range; points, outliers. P values were determined by two-tailed Wilcoxon test. b Principal coordinate analysis (PCoA) of the uterine and vaginal microbiome of different health statuses. The significance of separation of two clusters was measured with the Adonis test (P < 0.05). c The Bray-Curtis (BC) distance of the microbial communities among individuals within the same group and between different groups. The violin with box plot shows the median and interquartile range, and the width of the violin represents the density distribution of the BC distance values. P values were determined by two-tailed Wilcoxon test. d The relative abundance of the dominant genera in the uterine and vaginal microbiome of different health statuses. The two bars on the left show the average relative abundance of each group. e The relative abundances of discriminatory bacteria enriched in women with chronic endometritis. Linear discriminant analysis (LDA) effect size (LEfSe) analysis was performed to identify discriminatory bacteria, with an absolute value of log LDA score >3.0. f The co-occurrence of the same bacteria between the uterine and vaginal microbiome. In the top panel, the correlation coefficient of each OTU (each point) between the uterine and vaginal microbiome was calculated in women with (Disease group, yellow point) and without (Health group, blue point) chronic endometritis, respectively. In the bottom panel, the difference in the correlation coefficient of each OTU between the Disease group and the Health group is shown in blue and yellow bars.