Fig. 4: Reversible cycles, kinetics, and half-lives of fluorescence of TCPE, SRB, and C153 loaded in the dissipative assembly and dynamic cell imaging for ASP-CS-SRB. | Nature Communications

Fig. 4: Reversible cycles, kinetics, and half-lives of fluorescence of TCPE, SRB, and C153 loaded in the dissipative assembly and dynamic cell imaging for ASP-CS-SRB.

From: Light-fueled transient supramolecular assemblies in water as fluorescence modulators

Fig. 4

a Reversible variation in the fluorescence of TCPE at 450 nm. b Reversible fluorescence variation in SRB at 583 nm for five cycles between the SMEH-CS and ASP-CS dissipative processes over time at 15 °C (optical power density: 15 mW/cm2). The blue parts represent the light-induced formation of ASP-CS, and the gray parts represent the dissociation process. c Variation in the fluorescence of TCPE at 450 nm. d Fluorescence variation in SRB at 583 nm during the formation of dissipative assembly under 420 nm irradiation with different optical power densities from 5 to 25 mW/cm2 at 15 °C. e Variation in the fluorescence of TCPE at 450 nm. f Variation in the fluorescence of SRB at 583 nm during the dissociation of dissipative assembly at different temperatures from 15 °C to 35 °C. g Comparison of thermal dissociation half-lives at different temperatures from 15 to 35 °C detected by UV-Vis absorbance at 424 nm of ASP-CS and the fluorescence emission of TCPE (at 450 nm), SRB (at 583 nm), and C153 (at 500 nm) during thermal dissociation. n = 3 independent experiments, with the bar data indicating mean ± SD. (λex(TCPE) = 325 nm, λex(SRB) = 560 nm and λex(C153) = 425 nm). h Confocal images of the dissociation process of ASP-CS-SRB in HepG2 cells at Ex. 558 nm, Em. 570–620 nm. ([SMEH]initial = 0.15 mM, [CS] = 40 μg/mL, [TCPE] = 0.01 mM, [SRB] = [C153] = 0.001 mM, scale bar = 20 μm).

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