Fig. 6: Effects of mechanosensitive channel blockers on channel activities of MCA2(1-173). | Nature Communications

Fig. 6: Effects of mechanosensitive channel blockers on channel activities of MCA2(1-173).

From: MCAs in Arabidopsis are Ca2+-permeable mechanosensitive channels inherently sensitive to membrane tension

Fig. 6

a Negative pressure was applied before (left) and after (right) the application of 30 µM GdCl3. Current (top trace) and pressure (lower trace) are shown. The holding potential was 140 mV. b, c Changes in the open probability with pressure (b) and voltage (c) when 0 (black), 10 (blue), or 100 (red) µM GdCl3 was applied. d, e Changes in the open probability with pressure (d) and voltage (e) when 0 (black) or 5 (red) µM GsMT4 was applied. f Current traces before (left) and after (right) the application of LPC. The membrane was broken at the end of recording. The holding potential was 140 mV. In b, c, d, and e, the mean and standard deviation are shown as squares and bars. Small circles indicate individual data. Data from 5 (b), 3 (c), 4 (d), and 3 (e) patches. In b and d, the p-values of the two-sided t-test between the data obtained in the presence of blockers and those obtained in their absence are indicated.

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