Fig. 4: Enzyme-mimetic ROS production activity.
From: Hedgehog artificial macrophage with atomic-catalytic centers to combat Drug-resistant bacteria
a POD-mimetic activity via TMB-based UV–vis spectra in the presence of Art Ms and H2O2 (n = 3 independent experiments, data are presented as mean ± SD). b Typical Michaelis-Menten curves. For V-Art M7,5, V-Art M10, n = 3 independent experiments, data are presented as mean ± SD. The rest experiments were repeated three times and show a consistent trend. c Vmax, V0, Km, and TON values of V-Art M or Fe-Art M. d Comparison of the TON and Vmax values with reported enzyme-mimetic catalysts. NPs: nanoparticles; MOFs: metal-organic frameworks. e DMPO spin-trapping EPR spectra. f DMPO spin-trapping EPR spectra of Fe-Art M with different radical scavengers in DMSO solution. Benzoquinone (BQ, •O2− quencher), tertiary butanol (TBA, •OH quencher), NaN3 (1O2 quencher). g, h Detection of HPO-mimetic activity using CB as agents, the relative activity is referred to the rate of Intensity512 nm/Intensity648 nm. (In h, n = 3 independent experiments per group, data are presented as mean ± SD). In a, h, p values are assessed by unpaired Student’s two-sided t-tests. V is the initial velocity, Vmax is the maximal reaction velocity, Km is the Michaelis constant, TON is turnover number. In a, g, h, a.u. indicates the arbitrary units. Source data are provided as a Source Data file.
