Fig. 2: Genetic organization and verification of the angustmycin biosynthetic gene cluster.
From: Efficient biosynthesis of nucleoside cytokinin angustmycin A containing an unusual sugar system
a Genetic organization of the angustmycin biosynthetic gene cluster. The agm gene cluster is responsible for angustmycin biosynthesis in S. angustmyceticus JCM 4053 and S. decoyicus NRRL 2666. The two gene clusters in different strains harbor significant homologies, and the gray shaded bars represent the identities of the two corresponding enzymes encoded by the two gene clusters. b Bioassays of the metabolites produced by related recombinants of S. coelicolor M1154. The indicator strain is Mycobacterium smegmatis mc2155. The Roman numerals I, II, III, IV, and V represent JCM 4053, pCHW501, pCHW501ΔagmF, pCHW501ΔagmR, and pSET152, respectively, and the denotations of them correspond to those shown in (c). c HPLC analysis (λ = 254 nm) of the metabolites produced by related recombinants of S. coelicolor M1154. JCM 4053, 1 and 2 produced by S. angustmyceticus JCM 4053 as positive controls ; pCHW501, the metabolites of the recombinant S. coelicolor M1154 containing pCHW501; pCHW501ΔagmF, the metabolites of the recombinant S. coelicolor M1154 containing pCHW501ΔagmF; pCHW501ΔagmR, the metabolites of the recombinant S. coelicolor M1154 containing pCHW501ΔagmR; pSET152, the metabolites of the recombinant S. coelicolor M1154 containing pSET152 as a negative control. Allulose 6-P, Allulose 6-phosphate.
