Fig. 4: Arp8, but not Nhp10, regulates INO80-mediated spacing in vivo.

a Cartoon of the INO80 complex bound to the nucleosome based on PDB code 6FML https://www.rcsb.org/structure/6fml. The Nhp10 and Arp8 modules interact with linker DNA. b Deletion of NHP10 (blue lines) does not substantially alter the NRL distribution in TKO-Pol II samples (red; all colored lines peak at 167 bp). Cells depleted for INO80 are shown as a reference (grey). c Box plots of measured NRLs in the indicated single and double mutants in otherwise WT cells. Horizontal line is at 167 bp. P values are from a two-tailed paired Welch’s t-test on the mean values of two replicates compared to WT. d Same as b, but for arp8Δ. The NRL distribution shifts left peaking at 151 bp. e ARP8 deletion affects the amplitude and NRL of arrays. f ARP8 deletion shifts the NRL distribution to shorter values (peaks at 163 bp for arp8Δ and 166 bp for WT cells). This effect can be faithfully rescued by expression of wild-type Arp8 but not Arp8 lacking amino acids 2–197 (dashed lines). pEmpty: empty vector. Central line in box plot indicates the median, the box shows the interquartile range, and whiskers indicate data points within 1.5 times of the interquartile range.