Fig. 1: Representative 2-(2-phenylethyl)chromone skeletons and the identification of PECPS. | Nature Communications

Fig. 1: Representative 2-(2-phenylethyl)chromone skeletons and the identification of PECPS.

From: Identification of a diarylpentanoid-producing polyketide synthase revealing an unusual biosynthetic pathway of 2-(2-phenylethyl)chromones in agarwood

Fig. 1

a The core structures of three groups of 2-(2-phenylethyl)chromones and enzymatic products 1 and 2. b HPLC chromatograms for the formation of the C6–C7–C6 scaffold of tetrahydrobisdemethoxycurcumin (1) from 4-hydroxyphenylpropionyl-CoA and malonyl-CoA by PECPS (i), The formation of the C6–C5–C6 scaffold of 5-(4-hydroxyphenyl)-1-phenylpentane-1,3-dione (2) from benzoyl-CoA, malonyl-CoA, and 4-hydroxyphenylpropionyl-CoA by PECPS (ii) and boiled PECPS (iii). c Western blot analysis of the PECPS protein expression levels in 150 mM NaCl-treated A. sinensis calli (NaCl+) and healthy A. sinensis calli (NaCl-) at different time points. GAPDH was used as internal reference. d Western blot quantification of the relative expression levels of PECPS by ImageJ and normalization to the protein levels of GAPDH. Data represent the mean ± SD (n = 3). Statistical analysis was performed with unpaired two-tailed Student’s t-tests. Significance is shown with asterisks: ****p < 0.0001, ***p < 0.001, **p < 0.01. Exact p-values and source data are provided in the source data file. Total protein was extracted from 100 mg of calli using Plant Total Protein Extraction Reagent (Invent, USA) according to the manufacturer’s instructions. The protein expression levels of PECPS were analyzed by Western blotting with an anti-PECPS antibody prepared in rabbits.

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