Fig. 5: Detection of respiratory syncytial virus (RSV) in a one-step homogenous immunoassay by DIAMOND.
From: Digital plasmonic nanobubble detection for rapid and ultrasensitive virus diagnostics
a The schematic illustration of a homogeneous immunoassay for RSV utilizing antibody-conjugated AuNPs as probes at room temperature (RT). b TEM images of AuNP probes targeting RSV. The boundaries of RSV were highlighted in red. c Colorimetric analysis of the AuNP-based homogeneous immunoassay for different respiratory viruses. hMPV is Human metapneumovirus, PIV is Parainfluenza viruses, and IVA is Influenza A. d Bivariate plot of amplitude and AUC extracted from 3000 pulses for the assay solutions with RSV at different titers (PFU/mL). Dashed lines indicate the positions of thresholds calculated from the control sample. e Quantification of RSV titers as a function of fon. Inset shows the linear detection range. Error bars in (c) and (e) indicate the standard deviations of three independent measurements, the data were fitted by logistic fitting, and the LOD was calculated as 3 standard deviations of the control divided by the slope of regression line.
