Fig. 6: Olig2 recruits SETDB1 to silent inhibitors of oligodendrocyte differentiation.
a, b Scatterplot showing differentially expressed transcripts between iOL from Olig1-Cre; Setdb1F/+ and Olig1-Cre; Setdb1F/F brain cortex (a) and between rat OPCs transfected with Olig2 and Olig2-VP64 under differentiation condition (b). c, d Expression of well-known inhibition factors to rat OPC differentiation. Error bars indicate SEM (***p < 0.001; Hes1 in (d), p = 0.0029. two-tailed unpaired Student’s t test). e Efficiency of Sox11 knockdown in rat OPCs. Error bars indicate SEM (***p < 0.001; Hes1 in Olig2-VP64, p = 0.0029. one-way ANOVA). f Expression of Enpp6, Mbp and Plp1 was measured in rat OPCs under differentiation condition following transfection with indicated siRNA. Error bars indicate SEM (p < 0.001. one-way ANOVA). g Immunolabeling of CNP and OLIG2 in OPCs under differentiation condition following transfection with indicated siRNA. n = 3 independent experiments. h Genome browser visualization of OLIG2 or H3K9me3-targeting regions on the gene loci of Sox11 during rat OPC differentiation. i Luciferase activity of Sox11 promoter-driven reporters in control (Scramble) or Setdb1 knockdown (Setdb1 K/D) 293T cells transfected with indicated plasmids. Error bars indicate SEM (***p < 0.001; OLIG2+ in Scramble, p = 0.0078; SETDB1+ in Scramble, p = 0.0095. one-way ANOVA). j Immunolabeling for MBP and CC1 on the corpus callosum from Olig1-Cre; Setdb1F/+ and Olig1-Cre; Setdb1F/+; Sox11 F/F mice at P14. n = 3 control and three mutant mice. k, l Quantification of CC1+ oligodendrocytes density (k) and the percentage of MBP+ area (l) in corpus callosum of control and Sox11 mutant mice. Error bars indicate SEM (k, p = 0.0064; l, p = 0.0052. two-tailed unpaired Student’s t test). Scale bars: 50 μm in (g, j).
