Fig. 4: Evaluation of F3UTER predictions on an independent ER dataset using 3’-seq. | Nature Communications

Fig. 4: Evaluation of F3UTER predictions on an independent ER dataset using 3’-seq.

From: Leveraging omic features with F3UTER enables identification of unannotated 3’UTRs for synaptic genes

Fig. 4

a Schematic describing the framework of the process implemented to evaluate the performance of F3UTER on ERs in B cells. b Barplot showing the positive predictive value (PPV) and false omission rate (FOR) of 3’UTRs predicted by F3UTER, APARENT, GETUTR and TAPAS. The bars represent the average value across the four cell types (CD5, GCB, MB and NB), while the error bars show the standard deviation. The data points show the exact value of PPV and FOR in each cell type. c Genome browser view of the MTF2 locus, showing unannotated 3’UTRs detected downstream of MTF2 in GCB cells, RNA-seq expression in bigwig format (.bw), poly(A) sites in GCB, and the human genome mappability scores from UCSC (Umap). Umap S50 and S100: Single-read mappability for 50- and 100-mers; Umap M50 and M100: Multi-read mappability for 50- and 100-mers.

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