Fig. 4: Verification of the functional importance of PbHARP dimerization. | Nature Communications

Fig. 4: Verification of the functional importance of PbHARP dimerization.

From: Crystal structures and insights into precursor tRNA 5’-end processing by prokaryotic minimal protein-only RNase P

Fig. 4

A Interactions involved in PbHARP dimerization in the apo-form structure. B, C Interactions involved in PbHARP dimerization in the pre-tRNA-complexed structure. D Size-exclusion chromatographic analysis of wild-type, L103E/V107E and A118E/A121E mutants of PbHARP. E In vitro pre-tRNAHis cleavage assays catalyzed by WT PbHARP, L103E/V107E and A118E/A121E mutants. The substrate cleavage percentage (%) is shown at the bottom of the gels. Experiments were repeated independently three times with similar results. Source data are provided in Source Data file.

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