Fig. 5: Pre-tRNAHis mutation and cleavage by HARP. | Nature Communications

Fig. 5: Pre-tRNAHis mutation and cleavage by HARP.

From: Crystal structures and insights into precursor tRNA 5’-end processing by prokaryotic minimal protein-only RNase P

Fig. 5

A Sequence and secondary structure of 5’-end, acceptor stem and 3’-end of the native and mutated pre-tRNAHis. B In vitro cleavage assays using 1 μM pre-tRNA and 0.05 μM PbHARP. C In vitro cleavage assays using 1 μM pre-tRNA and 0.05 μM TrHARP. The substrate cleavage percentage (%) is shown at the bottom of the gels. Experiments were repeated independently three times (B, C) with similar results. Source data are provided in Source Data file.

Back to article page