Fig. 1: TIGIT directly binds C. albicans yeast and hyphae cells. | Nature Communications

Fig. 1: TIGIT directly binds C. albicans yeast and hyphae cells.

From: Candida albicans evades NK cell elimination via binding of Agglutinin-Like Sequence proteins to the checkpoint receptor TIGIT

Fig. 1

a Flow cytometry staining of C. albicans SC5314 yeast cells using TIGIT-Ig (Black empty histogram) or a negative control protein (filled gray histogram). One representative experiment out of 6 is presented. b Quantification of the results presented in (a). n = 6 independent experiments. c ELISA results of plate-bound C. albicans cells stained with TIGIT-Ig or a negative control protein. n = 3 independent experiments. d Quantification of flow cytometry stainings of various fungal species using TIGIT-Ig or a negative control protein. n = 2 independent experiments. e Immunofluorescence microscopy images of C. albicans yeast cells stained for their cell wall proteins with FITC (green) and either TIGIT-Ig or a negative control protein (red). f Immunofluorescence microscopy images of C. albicans hyphae cells stained for their cell wall proteins with FITC (green) and either TIGIT-Ig or a negative control protein (red). For figures (e, f) representative images from two independent experiments are presented. Data for (b–d) are presented as mean values ± SEM. Significance was tested using a two-tailed Student’s t test. ns = not-significant, * = p < 0.05, ** = p < 0.01.

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