Fig. 6: Als-mediated immune evasion can be prevented using TIGIT-targeted immunotherapy in mice. | Nature Communications

Fig. 6: Als-mediated immune evasion can be prevented using TIGIT-targeted immunotherapy in mice.

From: Candida albicans evades NK cell elimination via binding of Agglutinin-Like Sequence proteins to the checkpoint receptor TIGIT

Fig. 6

a Survival of mice infected with 6.5 × 10^5 CFU/mouse of the WT C. albicans strain SC5314 (black line) or a mutant strain deleted for ALS6 (blue line), ALS7 (green line), or ALS9 (red line). Each line represents 7–28 mice from 2–5 independent experiments. b C. albicans burden in the kidneys 48 h post I.V. infection of C57BL/6 mice. The mice were infected with either WT or ALS6, ALS7 or ALS9-deleted fungal cells and either treated or not with a TIGIT-blocking antibody. Kidneys were harvested, processed, and seeded on Sabouraud dextrose agar plates. n = 5–25 animals examined over 2–5 independent experiments. Data are presented as mean values ± SD. c Survival of mice infected with 6.5 × 10^5 CFU/mouse of the WT C. albicans strain SC5314 or a mutant strain deleted for ALS6, ALS7, or ALS9, and either mock treated (black line) or treated with a TIGIT-blocking antibody (red line). Each line represents 7–28 mice from 2–5 independent experiments. Data for the mock-treated mice is shared with (a). Error bars represent the standard error. For the fungal burden, significance was tested using a two-tailed Mann–Whitney test. For the survival assay significance was tested using Mantel–Cox log-rank test. ns = not-significant, * = p < 0.05, ** = p < 0.01.

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