Fig. 6: Validation of brain cell types and comparison of microglia regulome across species. | Nature Communications

Fig. 6: Validation of brain cell types and comparison of microglia regulome across species.

From: Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level

Fig. 6

a Schematic diagram visualizing the 9 different brain regions analyzed by this study. b t-SNE visualization of cells in the 9 brain regions. Cells were color-coded according to brain regions. Cell types were shown in extended Fig. S6. The hypergeometric test was used for GO term analysis, and p values were adjusted by Benjamini & Hochberg. c Representative IHC of SLC1A6, CALB2, PAX6, SLC17A7, and SST by antibody staining in the cortex or cerebellum. Arrows indicate corresponding cell types (n = 3). d Phylogenetic tree based on the NCBI taxonomy of animals used in this study (generated via http://www.timetree.org//). e Violin plots visualizing the expression of canonical microglia markers in the 13 species. f Conserved genetic regulatory networks in microglia within each indicated species. Light blue nodes represent regulators and green nodes represent corresponding target genes. g Feature plots visualizing the clustering of microglia single-cell transcriptome and expression of four TFs among the 13 species. Source data are provided as a Source Data file. Species icons in d, f were created with BioRender.com.

Back to article page